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Can a PCR detect TB and Cholera?

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We report a set of oligonucleotide primers and amplification conditions for the polymerase chain reaction to detect the ctx operon of Vibrio cholerae. The results of this ... viable cells. The polymerase chain reaction method could directly detect the ctx operon sequences in rice-water stool samples from patients with cholera. read more

PCR can be very effective at detecting infectious disease, given the extreme sensitivity of the technique. However, proper design plays a key role. There is always a risk of primers being unable to detect some variants of a pathogen (see NGS Saves A Young Life). read more

If you want to just see if they're present, you can just look for the 16S ribosomal rRNA sequence. This is just a sequence that is present in bacteria, but not other organisms like eukaryotes (amoeba, animals and plants) or viruses. read more

Suspected cholera case. In areas where a cholera outbreak has not been declared: Any patient 2 years old or older presenting with acute watery diarrhea and severe dehydration or dying from acute watery diarrhea. In areas where a cholera outbreak is declared: any person presenting with or dying from acute watery diarrhea. read more

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