Do eukaryotic cells have restriction endonucleases?

A different class of enzymes called exonucleases cut the DNA backbone, but only from the ends - either from the 3' end or the 5' end. MOST restriction endonucleases are prokaryotic in origin. However, there are several found in eukaryotic cells, including our own. In eukaryotes they are not referred to as restriction enzymes, just endonucleases. read more

No, eukaryotic cells do not have restriction endonucleases. All the restriction endonucleases have been isolated from various strains of bacteria. Prokaryotes/ bacteria have this enzyme as a defence mechanism to destroy the foreign DNA or to restrict the growth of bacteriophages. read more

No, eukaryotic cells do not have restriction endonucleases. This is because the DNA of eukaryotes is highly methylated by a modification enzyme, called methylase. Methylation protects the DNA from the activity of restriction enzymes .These enzymes are present in prokaryotic cells where they help prevent the invasion of DNA by virus. read more