Do owls hoot when flying?

Most of the time, no. They don't have a reason to, as it wastes their breath and alerts their prey. If their flying towards an intruding owl or a threat to their nest, then they might to scare it away. But owls usually hoot or make whatever noise they do in a tree instead (most owls don't hoot. A lot screech). read more

Besides territorial hooting, owls will sometimes make a hoot if startled by something. They lightly sleep during the day, but at the transitional times after dawn and right before dusk, they may just be waking up. read more

Owls are most often seen and heard during the night, because they are nocturnal. However, some owls, such as the pygmy owl, are active at twilight. And if you see or hear an owl during the day, it means that you are likely encountering a diurnal owl that sleeps at night just like we do—two diurnal owls are the burrowing owl and the short-eared owl. read more