Glycolysis itself does not use oxygen. Glycolysis itself is the splitting of glucose into two molecules of pyruvic acid. Glycolysis occurs in virtually all living creatures, including all animals, all plants and almost all bacteria. read more
Glycolysis takes place in two ways: aerobic with oxygen and anaerobic without oxygen. Aerobic glycolysis is the first stage of cellular respiration. During this 10-step process, oxygen plays a role in breaking down a single molecule of glucose to produce three ATP, two pyruvic acid and two Nicotinamide adenine dinucleotide. read more
No it does not. Glycolysis is a phase of aerobic respiration but it is also a phase of anaerobic respiration. Remember, all glycolysis is doing is producing 2 x pyruvate. Depending on how in-depth you studied the steps, O2 is never needed. read more