Yes and No, she's nearly always alive in the comics, though there is an alternate universe where she dies of cancer [1]. read more
There was also one occasion in 2000 where she appeared to die in an airplane explosion: Mary Jane is flying across America when her airplane explodes in midair in Amazing Spider-Man (Vol. 2) #13 (January 2000). But that turned out to be a villain’s plan to get at Parker, and she was later found to be alive. read more
In the series finale, Madame Web promises Spider-Man the real Mary Jane will be found. Mary Jane Watson has a minor appearance in Spider-Man Unlimited, voiced by Jennifer Hale. Mary Jane Watson appears in Spider-Man: The New Animated Series voiced by Lisa Loeb. She appears as the on-and-off girlfriend of Peter Parker. read more
Mary Jane Watson was the guiding light in Peter Parker's life, and their love had overcome countless obstacles. But her upbringing certainly gave her no reason to believe in happiness. Her parents, Madeline and Philip, met at high school and married aged eighteen. read more
If the player is defeated in battle, then both she and Spider-Man die. Mary Jane Watson appears in Spider-Man's endings in Capcom's Marvel Super Heroes and Marvel Super Heroes vs. Street Fighter. Mary Jane Watson appears in the Spider-Man game for the Dreamcast, Nintendo 64, and PlayStation, voiced by Jennifer Hale. read more