Mickey and Minnie were both created in 1928 and debuted on May 15 in "Plane Crazy" (http://en.wikipedia.org/wiki/Plane_Crazy), the first Mickey Mouse cartoon released (albeit without sound). read more
Thus if you believe that there is a soul in everybody, then yes Mickey has a soul. And by the way, the concept of soul cannot be attributed to only humans. There are animals out there who can feel misery, pain and suffering. read more