How do we isolate white blood cells from whole blood?

Fractionate the whole blood by centrifuging at 1500-2000 X g for 10-15 min at room temperature. This will separate the blood into an upper plasma layer, a lower red blood cell (RBC) layer, and a thin interface containing the WBCs (see Figure 1). read more

To isolate white blood cells (WBCs) from whole blood using Ficoll Histopaque, follow these steps: Remove proper media (such as, RPMI 1640) and histopaque solution from fridge and leave to warm at room temp . Ficoling must be performed in a suitable Tissue Culture. read more

The further isolation of white blood cells from bufy coat is quiet easy, for example You can centrifugute down the whole bufy coat with 1500 g, 10 min., pick them up in PBS, then use Ficol-paque separation (layer the cell containing PBS to the Ficol) and spin them with e.g. 1000 g 15-20 min (with slow acceleration and desceleration), where the neutrophils goes to the sediment, while monocytes and lymphocytes will be on the top of the Ficol (and under the PBS), in solution. read more