How to read histograms in flow cytometry?

To display data from a single parameter, we can use a univariate histogram (Figure 1.1). We can show the correlation between two parameters using a bivariate histogram, or cytogram, in the form of a dot, contour or density plot (Figure 1.2). read more

Back to Flow Cytometry Index Two-Parameter or Bivariate Histograms > Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents. read more

If the flow cytometer can sort cells, the computer controls the sorting process. As data are acquired, they written to the hard drive to create a file of data, often referred to as ‘listed data’. The computer program can then be used to analyse data subsequent to its acquisition; off-line analysis is useful for the preparation of illustrations for publications, lecture slides, etc. read more

Histogram - levels of one parameter (1D). Here the parameter is blue colour. There are two peaks on the histogram: blue-positive (right) and blue-negative (left) peak. The left peak is bigger than right peak, so we can conclude that there is more blue-negative cells, than blue-positive cells in the sample. read more