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Is E. coli K-12 MG1655 resistant to ampicillin?

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E. coli strain MG1655 was obtained by curing K-12 wild-type strain using acridine orange and UV. Yes, this strain is resistant to the antibiotic ampicillin. Source : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204738/pdf/jbacter00053-0167.pdf. read more

The high-level resistance phenomenon was found in biofilms of E. coli harboring pBR322 in the presence of subinhibitory concentrations of ampicillin and tetracycline, which confer resistance to penicillin, erythromycin, and chloramphenicol but not to kanamycin and gentamicin. read more

Y. Kang, T. Durfee, J. D. Glasner, Y. Qiu, D. Frisch, K. M. Winterberg, and F. R. Blattner (2004)."Systematic mutagenesis of the Escherichia coli genome." J Bacteriol 186: 4921-30. Construction of insertion alleles . We are constructing a set of mutant strains for MG1655. read more

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