What are considered the limitations of a light microscope?

Light is a major limitation of a light microscope. More specifically, the wavelength of the light waves that illuminate the specimen limits the resolution. The wavelength of visible light ranges from about 400 to 700 nanometers. read more

A: Light microscope disadvantages include limited resolution, lower magnification and poorer surface view of the specimen. Compound light microscope require the user to adjust to optical inversion, meaning the specimen will appear to be displaced in the opposite direction it is moved. read more

So light microscope is technically blind for specimen that smaller than visible wavelength. 535 Views Akash Verma, Have been studying logic and science for a decade. read more