Light is a major limitation of a light microscope. More specifically, the wavelength of the light waves that illuminate the specimen limits the resolution. The wavelength of visible light ranges from about 400 to 700 nanometers. read more
Visible light restricts the amount of resolution achieved with a light microscope. Magnification may range from 500x to 1500x, which is much smaller in comparison to electron microscopes; these give magnifications of 160,000x or more. read more