What is the purpose of pcr?

To do PCR, the original DNA that one wishes to copy need not be pure or abundant. It can be pure but it also can be a minute part of a mixture of materials. read more

The purpose of PCR is to amplify small amounts of a DNA sequence of interest so it can be analyzed separately. PCR can be used to make a large amount of a specific piece of DNA or to test a DNA sample for that sequence. read more

RT-PCR (Reverse transcriptase-polymerase chain reaction) is a highly sensitive technique for the detection and quantitation of mRNA (messenger RNA). The technique consists of two parts: The synthesis of cDNA (complementary DNA) from RNA by reverse transcription (RT) and ; The amplification of a specific cDNA by the polymerase chain reaction (PCR). read more