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Why does DNA precipitate in ethanol?

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After precipitation the nucleic acids can then be separated from the rest of the solution by centrifugation. The pellet is washed in cold 70% ethanol then after a further centrifugation step the ethanol is removed, and the nucleic acid pellet is allowed to dry before being resuspended in clean aqueous buffer. read more

SOLUBILITY : DNA (or RNA or any nuleic acid) is a polar molecule. read more

Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. The basic procedure is that salt and ethanol are added to the aqueous solution, which forces the precipitation of nucleic acid nucleic acids out of solution. read more

Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists. read more

If enough ethanol is added, the electrical attraction between phosphate groups and any positive ions present in solution becomes strong enough to form stable ionic bonds and DNA precipitation. This usually happens when ethanol composes over 64% of the solution. read more

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