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Will Triton X-100 'wash' off covalently bound antibodies?

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Non-ionic detergents such as NP-40 and Triton X-100 are less harsh than ionic detergents such as SDS and sodium deoxycholate. Other variables that can affect .... SDS buffer is the harshest, which will also elute non-covalently bound antibodies and antibody fragments along with the protein of interest. read more

Triton X-100 won't break covalent bonds. However, antibodies aren't usually covalently bound, just bound very strongly. Nonetheless, Triton won't wash off antibodies if used at normal concentrations (below 2%). read more

SDS buffer is the harshest, which will also elute non-covalently bound antibodies and antibody fragments along with the protein of interest. On the other hand, glycine buffer gently elutes the protein with reduced amount of eluted antibody. read more

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