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Types of Mucins

Acid-Complex Connective Tissue
Acid-Complex Connective Tissue

Mucin stains: Alcian blue: Stains acid-simple non-sulfated and acid-simple mesenchymal mucins at pH 2.5, acid-complex sulfated mucins at pH 1.0 and acid-complex connective tissue mucins at pH 0.5

Acid-Complex Sulfated: Found in Adenocarcinomas
Acid-Complex Sulfated: Found in Adenocarcinomas

Acid-complex sulfated: Found in adenocarcinomas Usually positive for PAS, ... acid-complex sulfated mucins at pH 1.0 and acid-complex connective tissue mucins at pH 0.5

Acid-Simple Mesenchymal
Acid-Simple Mesenchymal

Mucin stains: Alcian blue: Stains acid-simple non-sulfated and acid-simple mesenchymal mucins at pH 2.5, acid-complex sulfated mucins at pH 1.0 and acid-complex connective tissue mucins at pH 0.5

Acid-Simple non-Sulfated: Contain Sialic Acid
Acid-Simple non-Sulfated: Contain Sialic Acid

contain sialic acid, found in epithelium (gallbladder [benign, adenocarcinoma], intestinal metaplasia in stomach) Acid-simple non-sulfated mucins- stains positive for PAS, Alcian blue at pH 2.5, colloidal iron, and metachromatic dyes.

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