Is there any specific media for Gram-negative bacteria?

Eosin Methylene Blue agar (EMB agar) contains the dyes eosin and methylene blue. They inhibit Gram-positive organisms. Such a medium is selective for Gram-negative species. Lactose-fermenting organisms such as E. coli produce a black precipitate on EMB. read more

Few of them are available in any Microbiology Lab. It depends on kind of strain to isolate. We can start with standard agar, differential, and then selective agars. read more

Media must be prepared in such a way that it is sterile prior to being inoculated with a bacterial sample, so that, when a particular type of bacteria is cultured (cultivated) on that medium, it is the only type of bacteria present. read more

Plate A is a PEA plate with a Gram positive organism on the left and a Gram negative organism on the right. Plate B is a nutrient agar plate illustrating normal growth of each of the same bacteria. Notice that the Gram-negative organism shows inhibited growth on the PEA plate. read more